Transplantation of living nuclei from blastula cells into enucleated frogs' eggs

Transplantation of Living Nuclei From Blastula Cells into Enucleated Frogs' Eggs.

"Transplantation of Living Nuclei from Blastula Cells into Enucleated Frogs' Eggs" (1952), by Robert Briggs and Thomas J. King

In 1952 Robert Briggs and Thomas J. King [5] published their article, ?Transplantation of Living Nuclei from Blastula Cells into Enucleated Frogs? Eggs,? in the Proceedings of the National Academy of Sciences [6], the culmination of a series of experiments conducted at the Institute for Cancer Research [7] and Lankenau Hospital Research Institute [8] in Philadelphia, Pennsylvania. In this paper...

Transplantation of Living Nuclei of Late Gastrulae into Enucleated Eggs of Rana pipiens

I N previous papers it has been shown that living nuclei of animal hemisphere cells of frog blastulae and early gastrulae can be transferred into enucleated eggs (Briggs & King, 1952,1953a). Approximately 20 per cent, to 40 per cent, of the recipient eggs cleave normally, and the majority of these develop into complete embryos, demonstrating that the transplanted nuclei are undifferentiated (i....

The transplantation of nuclei from single cultured cells into enucleate frogs' eggs.

Nuclei from a monolayer of cultured epithelial cells have been transplanted singly to enucleated unfertilized eggs of Xenopus laevis. About three-quarters of the injected eggs failed to cleave or cleaved abortively. Most of the remainder formed partial blastulae. Less than 1 % reached postneurula stages of development. Nuclei from first-transfer blastulae were used for serial transplantation. T...

Methods of transplanting nuclei from single cultured cells to unfertilized frogs' eggs.

Two methods of transplanting single nuclei from monolayers of cultured cells to unfertilized eggs of Xenopus laevis are described, illustrated, and tested. The detached-cell method is simpler and quicker to operate and is suitable for homogeneous populations of cells which are easily removed from the substrate on which they are growing. The other, attached-cell, method is technically more elabo...